qPCR Calculator

qPCR Calculator is an Edge browser extension designed for molecular biology researchers, aimed at simplifying the analysis process of real-time quantitative PCR (qPCR) data.
1. **打开插件 / Open Plugin**
- 点击浏览器工具栏中的插件图标，打开qPCR Calculator的主界面
Click the plugin icon in the browser toolbar to open the main interface of qPCR Calculator
- 或者在任何页面右键，选择"qPCR Calculator" > "打开主界面"
Or right-click on any page, select "qPCR Calculator" > "Open Main Interface"

2. **选择孔板类型 / Select Plate Type**
- 在主界面中，选择您使用的孔板类型（96孔板或384孔板）
In the main interface, select the plate type you are using (96-well or 384-well)

3. **添加样本属性 / Add Sample Properties**
- **通过粘贴导入 / Import via Paste**：从Excel或CSV复制的数据格式，插件会自动识别并分配到对应的孔位
Copy data from Excel or CSV, the plugin will automatically recognize and assign it to the corresponding wells
- **通过输入分配 / Assign via Input**：点击孔板上的特定孔位，弹出属性编辑窗口，填写样本名称、基因名称、CT值、重复编号等信息
Click on a specific well on the plate to open the property editing window, fill in sample name, gene name, CT value, replicate number, etc.

4. **确定复孔 / Determine Replicates**
- 相同样本名称和基因名称的孔位会自动识别为复孔
Wells with the same sample name and gene name will be automatically recognized as replicates
- 您可以在孔板上手动选择需要组合为复孔的孔位
You can manually select wells on the plate to be combined as replicates
- 插件会自动计算复孔的平均CT值，提高数据可靠性
The plugin will automatically calculate the average CT value of replicates, improving data reliability

5. **设置对照和参考基因 / Set Control and Reference Gene**
- 选择对照样本和参考基因，这些将用于ddCt值的计算
Select control samples and reference genes, which will be used for ddCt value calculation

6. **计算并查看结果 / Calculate and View Results**
- 点击"计算"按钮，插件将自动计算ddCt值和基因表达水平
Click the "Calculate" button, the plugin will automatically calculate ddCt values and gene expression levels